Table 1. Comparison of read statistics.

	LIGR-seq	PARIS	SPLASH
Total number of sequencing reads	171,239,817	99,698,824	189,340,955
Chimeric reads	6,614,251 (~3.9%)	2,077,743 (~2%)	1,038,801 (~0.5%)
RNA–RNA interactions	1,029	232,031a	4,026

Data derived from psoralen-treated human cell line samples (LIGR-seq and PARIS: total RNA isolated from HEK293T human embryonic kidney cells; SPLASH: total RNA from GM12892 human lymphoblastoid cells); all replicates included. Values are taken from the supplementary information of the respective publication (LIGR-seq ²¹, PARIS ²², and SPLASH ²³). In the case of LIGR-seq, the number of chimeric reads was determined by the corresponding analysis pipeline Aligater ²¹. ^a So-called Duplex Groups, representing gapped reads with interacting RNA sites. LIGR-seq, ligation of interacting RNA followed by high-throughput sequencing; PARIS, psoralen analysis of RNA interactions and structures; SPLASH, sequencing of psoralen-crosslinked, ligated, and selected hybrids.