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. Author manuscript; available in PMC: 2018 Nov 21.
Published in final edited form as: Cell Rep. 2018 Oct 23;25(4):1051–1065.e6. doi: 10.1016/j.celrep.2018.09.075

Figure 6. dTat Is Dispensable for NOMPC-Microtubule Interactions.

Figure 6.

(A) Maximum intensity projections showing GFP-NOMPC distribution in c3da neurons wild-type control (left) and dTatKO mutant (right) larvae additionally expressing the membrane marker CD4-tdTomato.

(B) S2 cells stably transfected with UAS-GFP-nompC were immunostained with antibodies to GFP and acTb or α-tubulin, as indicated. Images show cells in interphase (left, middle) and during anaphase (right).

(C) S2 cells stably transfected with UAS-GFP-nompC were treated with control RNAi, dTat RNAi, taxol, or tubacin; immunostained using GFP and tubulin antibodies; and the fraction of cells exhibiting NOMPC-microtubule co-localization was visually scored in a blind experiment. Chi-square tests revealed no differences in NOMPC-microtubule co-localization among the different treatments. The number of cells analyzed is shown for each treatment.