KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-Tubulin (clone DM1A) | Sigma-Aldrich | Cat# T9026; RRID: AB_477593 |
| Mouse monoclonal anti-Tubulin (clone 6-11B-1) | Sigma-Aldrich | Cat#T7451, RRID: AB_609894 |
| Mouse monoclonal anti-Tubulin (clone DM1A); FITC conjugate | Sigma-Aldrich | Cat# F2168, RRID: AB_476967 |
| Mouse monoclonal anti-Myc (clone 9E10) | Developmental Studies Hybridoma Bank (DSHB) | Cat# 9E 10, RRID: AB_2266850 |
| Mouse monoclonal anti-Futsch (clone 22C10) | DSHB | Cat# 22c10, RRID: AB_528403 |
| Rabbit polyclonal anti-GFP antibody | Fisher | Cat# A-11122, RRID: AB_22159 |
| Rabbit polyclonal anti-dsRed antibody | Clontech | Cat# 632496, RRID: AB_10013483 |
| Rabbit polyclonal anti-dTat antibody | This study | N/A |
|
Chemicals, Peptides, and Recombinant Proteins | ||
| Paclitaxel | Sigma-Aldrich | T7402 |
| Tubacin | Sigma-Aldrich | SML0065 |
|
Deposited Data | ||
| D. melanogaster larval PNS RNA-Seq data | Williams et al., 2016 | NCBI-SRA GSE72884 |
| D. melanogaster whole larva RNA-Seq data | Boiko et al., 2017 | NCBI-SRA GSE99711 |
| D. melanogaster larval cell type RNA-Seq data | This study | NCBI-SRA GSE120305 |
|
Experimental Models: Cell Lines | ||
| D. melanogaster: Cell line S2 | Derosophila Genomics Resource Center | S2-DGRC |
|
Experimental Models: Organisms/Strains | ||
| w[1118] ; dTat[KO] | This study | NA |
| w[1118] ; dTat[GFP] | This study | NA |
| w[1118] ; UAS-GFP-dTat-L | This study | NA |
| y[1] M{vas-Cas9.RFP-}ZH-2A w[1118]/FM7a, P{w[+mC] = Tb[1]}FM7-A | Bloomington Drosophila Stock Center (BDSC) | 55821 |
| y[1] w[67c23] P{y[+mDint2] = Crey}1b; sna[Sco]/CyO; Dr[1]/TM3, Sb[1] | BDSC | 34516 |
| w[1118] | BDSC | 6326 |
| w[1118] ; ppk-Gal4 | BDSC | 32079 |
| y[1] w[*] HDAC6[KO] | BDSC | 51182 |
| nompC[1] cn[1] bw[1]/CyO | BDSC | 42268 |
| nompC[3] cn[1] bw[1]/CyO | BDSC | 42258 |
| w[1118]; P{w[+mC] = AyGAL4}17b | BDSC | 4413 |
| y[1] w[*]; PBac{y[+mDint2] w[+mC] = nompC-GAL4.P}VK00014; Df(3L)Ly, sens[Ly-1]/TM6C, Sb[1] Tb[1] | BDSC | 36361 |
| w[*]; P{w[+mC] = UAS-TeTxLC.tnt}R3 | BDSC | 28997 |
| w*; P{UAS-Hsap\KCNJ2.EGFP}7 | BDSC | 6595 |
| w[*]; M{w[+mC] = UAS-Kat60.M}ZH-51D/CyO | BDSC | 64117 |
| w[1118]; P{w[+mC] = UAS-Eb1.EGFP.H}G | BDSC | 36861 |
| y[1] w[*]; P{w[+mC] = UAS-CD4-tdTom}7M1 | BDSC | 35841 |
| w[1118]; P{w[+mC] = ppk-CD4-tdTom}10a/TM6B, Tb[1] | BDSC | 35845 |
| w[1118]; Df(3L)BSC113/TM6B, Tb[1] | BDSC | 8970 |
| w[1118], ppk-mCD8-GFP | Jiang et al., 2014 | NA |
| w[1118] ; αTub84B[K40A] | Jenkins et al., 2017 | NA |
| w[1118]; aTub84B[K40R] | Jenkins et al., 2017 | NA |
| w[1118] ; αTub84B[Δ] | Jenkins et al., 2017 | NA |
| w[1118]; P{w[+mW.hs] = GawB}21-7 | Song et al., 2007 | NA |
| w[1118]; P{w[+mW.hs] = GawB}19-12 | Xiang et al., 2010 | NA |
|
Oligonucleotides | ||
| Oligonucleotide sequences used in this study are listed in Table S4. | This study | NA |
|
Recombinant DNA | ||
| pBID-UAS-GFP-dTat-L | This study | NA |
| pET28a dTat (1-196) | This study | NA |
| pMT B V5/His dTat(alt)-S | This study | NA |
| pMT B V5/His dTat(alt)-S GG (G133Y, G135Y) | This study | NA |
| pMT B V5/His dTat(alt)-S | This study | NA |
| pMT B V5/His dTat(alt)-L GG (G133Y, G135Y) | This study | NA |