Skip to main content
. 2017 May 31;38(7):728–737. doi: 10.1093/carcin/bgx049

Figure 3.

Figure 3.

LTA4H mediates p27 stability. (A) A431 cells were infected with the indicated shRNAs for 48 h and then cell distribution was analyzed by flow cytometry. (B) A431, SCC12, or SCC13 cancer cells were infected with the indicated shRNAs for 48 h and then whole cell lysates were analyzed by Western blotting to detect LTA4H or p27 protein expression. (C) A431 cells were infected with the indicated shRNAs for 48 h and then mRNA levels were analyzed by quantitative PCR (qPCR). Relative mRNA levels were normalized against gapdh. Data are shown as mean values ± S.D. (D) A431 cells expressing the indicated shRNAs were treated with 20 µg/ml cycloheximide (CHX) and harvested at the indicated time points. Whole cells lysates were analyzed by Western blot to detect LTA4H and p27. Relative protein levels were normalized against ß-actin and data are shown as mean values ± S.D. (*P < 0.05). (E) A431 cells expressing the indicated shRNAs were incubated with or without MG132 (10 µM) for 12 h. Whole cell lysates were analyzed by Western blotting as indicated.