Figure 2.

miR-125a-5p suppressed CRC cell proliferation, migration, invasion, and angiogenesis in vitro. (A) The expression of miR-125a-5p was upregulated in HCT116 and HT29 cells via transfecting with agomiR-125-5p. (B) CCK-8 assay was performed to assess the proliferation of HCT116 and HT29 after transfection with agomiR-125a-5p or negative control (agomiR-NC). (C, D) Wound healing and transwell assay were performed to detect the ability of migration and invasion of agomiR-125a-5p-transfected CRC cells or negative control. (E) HUVECs were cultured on Matrigel-coated plate with CM from CRC cells with agomiR-125a-5p or agomiR-NC transfection. Data are shown as mean±SD. *P<0.05, **P<0.01, ***P<0.001.

Abbreviations: CRC, colorectal cancer; CCK-8, cell counting kit-8; CM, conditioned medium; HUVEC, human umbilical vein endothelial cell.