Figure 1.

Med12^fl/fl Amhr2-Cre female mice are infertile. (A) Breeding crosses used to generate Med12^fl/fl Amhr2-Cre and Med12^fl/y Amhr2-Cre mice for experimental studies. (B) Schematic representation of floxed Med12 allele. LoxP sites bracket exons 1–7 of the Med12 gene. In Amhr2-Cre transgenic mice, Amhr2 promoter drives Cre expression in uterine mesenchyme, oviduct, and granulosa cells of ovaries. (C) Fertility data of control (Med12^fl/fl) (n = 7), Med12^fl/+ Amhr2-cre (n = 13), and Med12^fl/fl Amhr2-cre (n = 7) female mice. The mice were bred with wild-type stud males for 6 months. (D) Mating success was evaluated by vaginal plug detection (n = 8). Data are presented as mean ± SEM. ^***P < 0.001 (C, D).