Figure 1.

The biological structure of circ-SFMBT2. (A) Schematic diagram shows that circ-SFMBT2 is derived from SFMBT2 exon 5–8. The amplification products were used for Sanger sequencing to determine the correctness of circ-SFMBT2 primers. The divergent primers were designed to confirm the back-splice junction of circ-SFMBT2. (B) qRT-PCR for the abundance of circ-SFMBT2 and SFMBT2 mRNA in GC cells treated with RNase R. (C) qRT-PCR for the abundance of circ-SFMBT2 and SFMBT2 mRNA in GC cells treated with actinomycin.

Abbreviations: GC, gastric cancer; qRT-PCR, quantitative real-time PCR.