Figure 4.
GBS-stimulated PGE2 release from gestational membranes. Extraplacental membranes were obtained from pregnant donors and cultured in a transwell system with choriodecidua (CD) and amnion (AM) facing the inner and outer chambers, respectively. Twenty-four hours after inoculation with GBS (1 × 106 CFU/mL) media was collected from CD and AM compartments. Levels of PGE2 in media were then detected via ELISA assay. T-tests (P < 0.05) were used to identify significant differences in PGE2 levels between GBS-treated and control samples. N = 6 women (3 replicates/woman).