Figure 6.

Vigorous motility patterns are reduced significantly when human sperm are incubated under noncapacitating conditions. Sperm samples from eight individuals were split and incubated for 5 h in either HTF (capacitating, solid lines) or mHTF (noncapacitating, dashed lines) medium. Aliquots were assessed for motility at 1-h intervals, and motility profiles were determined by CASAnova. Bars represent the mean ± SEM of motile tracks. Differences between motility groups at corresponding time points were determined by two-tailed unpaired t-test after arcsine transformation of percentages. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.