Figure 2.

Metformin treatment does not compromise the development of placenta and embryos (A and B) Immunostaining of PECAM and in situ hybridization of Tpbpa on day 16 in WT and Faah^−/− implantation sites. Positive PECAM signals highlighted all endothelial cells, whereas Tpbpa signals are localized on spongy trophoblast cells. Three implantation sites from different females were examined, and a representative image for each staining is shown in the figure. (C) Litter size of WT and Faah^−/− mice with or without metformin treatment. (D and E) Body weights and survival rates of neonatal pups of WT and Faah^−/− mice with or without metformin treatment. All neonatal pups were derived from litters shown in panel C. Dec, decidua; Sp, spongy layer; Lab, Labyrinth zone; Veh, vehicle; Met, metformin.