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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: J Bone Miner Res. 2018 Jul 19;33(11):2021–2034. doi: 10.1002/jbmr.3530

Fig. 6.

Fig. 6.

Tsc1 deletion leads to mTORC1-dependent downregulation of Wnt/β-catenin signaling. (A) BMSCs were analyzed by Western blotting with indicated antibodies. (B) qPCR analysis of the mRNA expression of β-catenin target genes Axin2, Cnx43, and Lef1 in BMSCs. n = 3. (C) TSC1 CKO BMSCs were treated with rapamycin at indicated concentration for 24 hours and analyzed by Western blotting with indicated antibodies. (D) MEF TSC1+/+ and TSC1–/– cells were analyzed by Western blotting with indicated antibodies. (E) qPCR analysis of the mRNA expression of β-catenin target genes Axin2, Cnx43, and Lef1 in MEFs. n = 3. (F) MEFs were treated with rapamycin at 10 nM or 20 nM for 24 hours and analyzed by Western blotting with indicated antibodies. For B and E, *p < 0.05, values are shown as mean + SE. CTR = control (Tsc1F/F).