Skip to main content
. 2018 Nov 21;12(11):e0006854. doi: 10.1371/journal.pntd.0006854

Fig 1. EAPB0503 exhibits a higher efficacy on L. tropica amastigotes replication.

Fig 1

Real-time quantitative PCR detection of infected macrophages with L. major or L. tropica amastigotes treated with different concentrations of Imiquimod or EAPB0503 (A, B). RT-PCR detection of infected macrophages with L. major (C) or L. tropica (D) amastigotes treated with 0.1 μM of Imiquimod or EAPB0503 for 10 and 24h. Briefly, differentiated and activated THP-1 into macrophages were infected with L. major or L. tropica at the ratio of 5 parasites/cell for 24h. Treatment with 0.1, 0.5, 1 or 10 μM of Imiquimod or its analog EAPB0503 was performed for 24h (A-D). Treatment with 0.1μM of Imiquimod or EAPB0503 was performed for 10 and 24h (C, D). The results are shown as percentage of untreated infected macrophages. Amastigote transcripts were evaluated by Syber green RT-PCR using kinetoplast specific primers and their percentage of expression was normalized to GAPDH. Results are expressed as percentage of untreated control (±) SD and are representative of at least three independent experiments. Giemsa staining on untreated or treated macrophages infected with amastigotes of L. major (E) and L. tropica (F) strains. Treatment with 0.1 μM of Imiquimod or EAPB0503 was performed for 10 or 24h. The results depict one representative of three independent experiments. The t-test was performed to validate significance. *, ** and *** indicate p values ≤ 0.05; 0.01 and 0.001, respectively. P-values less than 0.05 were considered significant.