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. 2018 Nov 21;14(11):e1007792. doi: 10.1371/journal.pgen.1007792

Fig 3. mRNA fragments identified at the splice junction of Ll.LtrB circles.

Fig 3

Additional nts are shown along with their flanking sequences (5’ flanking) (3’ flanking), their origin (Gene name) and frequency of identification between parentheses for Ll.LtrB-ΔLtrA+LtrA (a) and Ll.LtrB-EBS1/Mut-ΔLtrA+LtrA (b) circles. The junctions between the additional nts and their flanking regions (/) as well as the IBS1- (yellow) and IBS2- (green) like sequences are denoted. The bolded nts represent residues from the IBS1- and IBS2-like sequences that can potentially base pair with the intron’s EBS1 and EBS2 sequences specified above. Sequences spanning two genes and including a short intergenic region are underlined. The genes in bold (alaS, enoA, S12/S7) were further studied for Ll.LtrB reverse splicing analyses and the detection of E1-mRNA and mRNA-mRNA chimeras (Figs 7 and 8).