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. Author manuscript; available in PMC: 2019 Apr 16.
Published in final edited form as: Angew Chem Int Ed Engl. 2018 Mar 14;57(17):4756–4759. doi: 10.1002/anie.201801167

Figure 3: Bicyclic peptides conjugated to PMO show increased exon-skipping activity.

Figure 3:

A) Depiction of bicyclic peptide conjugate PMO-3b. B) Conjugates between PMO and perfluoroaryl cyclic or bicyclic peptides (6c, 1b, and 3b) lead to more cellular fluorescence than conjugates to linear R12 (5), an established cyclic peptide cR10 (7c), or a non-fluorinated bicyclic peptide (1nfb). The PMO corrects eGFP splicing in a modified HeLa cell line. Cells were incubated with 2 μM or 5 μM of each PMO-peptide conjugate for 22 hours and the mean fluorescence intensity was analyzed by flow cytometry. Error bars are standard deviation (n=3 independent replicates).