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. 2018 Nov 21;4(11):eaar6280. doi: 10.1126/sciadv.aar6280

Fig. 2. Schematic representation of GNCIA in detection of HIV-1 p24 antigen.

Fig. 2

Capture antibodies are coated on the microtiter wells that capture the HIV-1 p24. Subsequently, secondary biotinylated anti-p24 antibodies, which bind to the captured p24 antigen, are added. The immunocomplex then attaches to the AuNC-SA, which recognizes and interacts with the above biotinylated sandwich complex. After multiple wash steps, the signal released from the wells is recorded with a SpectraMax M5 multiplate reader (27).