Table 2. A list of primers (Integrated DNA Technologies, IA, USA) and probes (Applied Biosystems, Foster City, CA, USA) used in the qPCR analysis).
DNA Target | Primer | Sequence (5'-3') | Reference |
---|---|---|---|
Microcystis 16s rDNA | 184F | GCCGCRAGGTGAAAMCTAA | [39] |
431R | AATCCAAARACCTTCCTCCC | [39] | |
Probe | (Taq) FAM-AAGAGCTTGCGTCTGATTAGCTAGT-BHQ-1a | [37] | |
Microcystis mcyD | F2 | GGTTCGCCTGGTCAAAGTAA | [40] |
R2 | CCTCGCTAAAGAAGGGTTGA | [40] | |
Probe | (Taq) FAM-ATGCTCTAATGCAGCAACGGCAAA-BHQ-1a | [37] |
F: forward primer, R: reverse primer.
a Black Hole Quencher-1 (quenching range 480–580 nm)