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. 2018 Nov 21;13(11):e0206821. doi: 10.1371/journal.pone.0206821

Table 2. A list of primers (Integrated DNA Technologies, IA, USA) and probes (Applied Biosystems, Foster City, CA, USA) used in the qPCR analysis).

DNA Target Primer Sequence (5'-3') Reference
Microcystis 16s rDNA 184F GCCGCRAGGTGAAAMCTAA [39]
431R AATCCAAARACCTTCCTCCC [39]
Probe (Taq) FAM-AAGAGCTTGCGTCTGATTAGCTAGT-BHQ-1a [37]
Microcystis mcyD F2 GGTTCGCCTGGTCAAAGTAA [40]
R2 CCTCGCTAAAGAAGGGTTGA [40]
Probe (Taq) FAM-ATGCTCTAATGCAGCAACGGCAAA-BHQ-1a [37]

F: forward primer, R: reverse primer.

a Black Hole Quencher-1 (quenching range 480–580 nm)