Table 1: PCR primer sequences used in qPCR.
Gene | Forward primer | Reverse primer | Tm (°C) | Reference |
---|---|---|---|---|
Car | GGAGCGGCTGTGGAAATATTGCAT | TCCATCTTGTAGCAAAGAGGCCCA | 56.5 | This manuscript |
Cpt1a | TTGATCAAGTGCCGGACGAGT | GTCCATCATGGCCAGCACAAAGTT | 55.5 | This manuscript |
Cyp2a4 | AGCAGGCTACCTTCGACTGG | GCTGCTGAAGGCTATGCCAT | 63.6 | (Wiwi et al., 2004) |
Cyp2b9 | CTGAGACCACAAGCGCCAC | CTTGACCATGAGCAGGACTCC | 64.3 | (Wiwi et al., 2004) |
Cyp2b10 | CTGAATCCGCTCCTCCACACTC | TGAGCCAACCTTCAAGGAATAT | 61.4 | (Hernandez et al., 2006) |
Cyp2b13 | GAACTGAGACTACCAGCACCACTCCT | TGAGCATGAGCAGGAAACCACT | 61.5 | (Wiwi et al., 2004) |
Cyp2c29 | GGCCTCAAAGCCCTACTGTCA | AACGCCAAAACCTTTAATC | 53.6 | (Luo et al., 1998) |
Cyp3a11 | CTTTCCTTCACCCTGCATTCC | CTCATCCTGCAGTTTTTTCTGGAT | 64.2 | (Wiwi et al., 2004) |
Foxa2 | TCAACGACTGCTTTCTCAAGGTGC | TTCTCGAACATGTTGCCCGAGTCT | 57.8 | (Wiwi et al., 2004) |
18s rRNA | CGCCGCTAGAGGTGAAATTC | CCAGTCGGCATCGTTTATGG | 51 | (Hernandez et al., 2006) |
Tm: Annealing Temperature