Table 5: qPCR measured expression of Cyp and metabolic transcription factors in young (9-week old) and old (35-week old) WT and Cyp2b-KD (KD) male mice.
Males | Untreated (9) weeks | Old 35-weeks | ||
---|---|---|---|---|
WT | KD | WT | KD | |
Cyp2b9 | 1.0 ± 0.28 | 0.29 ± 0.31** | 1.0 ± 0.43 | 8.76 ± 2.89 |
Cyp2b10 | 1.0 ± 0.31 | 0.33 ± 0.08 | 1.0 ± 0.26 | 2.12 ± 0.99 |
Cyp2b13 | 1.0 ± 0.30 | 0.21 ± 0.03*** | 1.0 ± 0.26 | 8.75 ± 5.43 |
Cyp2a4 | 1.0 ± 0.19 | 0.48 ± 0.12* | 1.0 ± 0.53 | 0.20 ± 0.03 |
Cyp2c29 | 1.0 ± 0.32 | 1.19 ± 0.41 | 1.0 ± 0.28 | 1.79 ± 0.5 |
Cyp3a11 | 1.0 ± 0.18 | 1.43 ± 0.64 | 1.0 ± 0.11 | 1.49 ± 0.48 |
Car | 1.0 ± 0.18 | 0.89 ± 0.24 | 1.0 ± 0.22 | 1.44 ± 0.54 |
Foxa2 | 1.0 ± 0.23 | 0.60 ± 0.12 | 1.0 ± 0.23 | 0.83 ± 0.21 |
Cpt1a | 1.0 ± 0.32 | 0.85 ± 0.29 | 1.0 ± 0.32 | 0.65± 0.41 |
Data are presented mean ± SEM normalized to untreated 9-week old mice. Significant changes in gene expression were determined by Student’s t-tests using the GraphPad Prism 4.0 software package (n = 5–6).
P value <0.05
< 0.01
< 0.001