Table 1:

Mouse models of regulatory ASD genes (CHD8, FOXP1, RBFOX1).

	Reference	Strategy Used	Morphological Phenot	Behavioral Phenotype	Physiological Deficits	Downstream Targets
CHD8	Katayama et al49, 2016	Heterozygous s knockout (Cre-mediated)	macrocephaly	Increased anxiety, deficits in social behaviour, normal learning	n.d.	neuronal development, REST complex
	Durak et al.51, 2016	in utero knockdown (in developing cortex)	Defective neural progenitor proliferation	deficits in social behaviour, normal learning	n.d.	cell cycle, Wnt signaling
	Platt et al.53, 2017	heterozygou s knockout (CRISPR-mediated)	macrocephaly	mild social defects, increased anxiety, no repetative behaviour, increased acquired motor learning	decreased inhibitory signaling in SPNs of Nac	chromatin remodelling, mRNA processing, cell cycle, Wnt signaling
	Gompers et al.52, 2016	heterozygou s knockout (CRISPR-mediated)	macrocephaly	normal social behaviour, no repetative behaviour, learning and memory impairment	n.d.	RNA processing, chromatin remodelling, cell cycle
	Suetterlin et al.50, 2018	heterozygou s knockout (Cre-mediated)	macrocephaly	normal social behaviour, delayed motor development, hypoactivity in adults	n.d.	CNS development, cell adhesion, axon guidance
FOXP1	Bacon et al.65, 2015	brain-specific knockout (Nestin.Cre-mediated)	enlarged lateral ventricle, abnormalitie s in striatum, decreased neuronal density in hippocampu s	increased repetitive behavior, decreased social interest and impairment s in patial memory	decreased excitability and increased excitatory synaptic transmission in hippocampal pyramidal neurons.	chromatin, nucleosome, cell cyle
	Araujo et al.66, 2015	heterozygous knockout	n.d.	defects in neonatal ultrasonic vocalizations	increased excitability of striatal SPNs	striatal development
	Li et al.67, 2015	in utero knockdown (in developing cortex)	defective neuronal migration, defective Neurite development	n.d.	n.d.	n.d.
	Araujo et al.68, 2017	conditional knockout in forebrain pyramidal neurons (Emx.Cre-mediated)	decreased hippocampal volume	hyperactivity, decreased sociability, impaired hippocampal-based spatial learning	decreased late-phase long-term potentiatio n (LTP) response	neurogenesis, neural differantiation, synaptic transmission
	Usui et al.69, 2017	conditional knockout in forebrain pyramidal neurons (Emx.Cre-mediated)	reduced neocortical size and mispositioning of deep layer neurons	impaired postnatal vocal communicatio n	n.d.	neurogenesi s and neuronal migration
RBFOX1	Gehman et al.77, 2011	brain-specific knockout (Nestin.Cre-mediated)	normal gross morphology	seizures	hyperexcitbility in hippocampal neurons	SNARE complex, neurotransmitter genes, ion channels
	Hamada et al.79, 2015	in utero knockdown (in developing cortex)	defects in neuronal migration, neuronal placement, and dendritic arbor formation	n.d.	n.d.	n.d.

SPN: spiny projection neuron, Nac: nucleus accumbens, n.d: not determined