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. 2018 Nov 21;8:17210. doi: 10.1038/s41598-018-35496-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FGF19-M52 analogue protects Fxr^−/− mice from fibrosis and cellular proliferation. (a) Liver histology assessed by hematoxylin and eosin (H&E) staining and observed by light microscopy (magnification, 10–20X). (b) Mcp-1 and Clec7a gene expression levels assessed by real time qPCR in tumor-free liver extracts. (c) Hepatic immunohistochemical staining of the oncogene Ccnd1. (d) Quantification of Ccnd1 staining assessed with ImageJ and expressed as % of the area occupied by positive cells. (e) Ccne1, p21 and c-myc gene expression levels assessed by real time qPCR in tumor-free liver extracts. Expression was normalized to Cyclophillin. (f) Hepatic collagen deposition assessed by Sirius Red staining (magnification, 10–20X). (g) Quantification of collagen deposition assessed with ImageJ and expressed as % of collagen staining/field (h) Col1a1 gene expression levels assessed by real time qPCR in tumor-free liver extracts. Expression was normalized to Cyclophillin. All values represent mean ± SEM. Statistical significance (**p < 0.01, ***p < 0.001) was assessed by Mann-Whitney’s U test.