Fig. 5.

Comparison of vitamin B₁₂ production and growth in E. coli strains expressing Module 4 and 5. a Vitamin B₁₂ production and growth of E. coli expressing the cobN,S,T genes from a single species (B. melitensis, S. meliloti or R. capsulatus) as well as the cobW gene from B. melitensis, S. meliloti, or R. capsulatus. b Vitamin B₁₂ production and growth of E. coli strains expressing various combinations of the cobN, cobS, cobT, and cobW genes from B. melitensis, S. meliloti, or R. capsulatus. c Vitamin B₁₂ production and growth of E. coli expressing different variant forms of Module 4. Bm, Sm, Rc, St, and Ec represent abbreviations of strains B. melitensis, S. meliloti, R. capsulatus, S. typhimurium, and E. coli, respectively. 1, 2, 3, 4, and 5 in Fig. 5c represent genes encoding cob(I)yrinic acid a,c-diamide adenosyltransferase, adenosylcobyric acid synthase, _L-threonine kinase, threonine-O-3-phosphate decarboxylase, and AdoCbi-P synthase, respectively. All strains were cultured in CM medium for vitamin B₁₂ production. Error bars indicate standard deviations from triplicate biological replicates