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. 2018 Nov 21;50(11):154. doi: 10.1038/s12276-018-0177-z

Fig. 3. The anti-apoptotic effect of melatonin is related to autophagy flux in AGE-treated EPCs.

Fig. 3

EPCS were untreated or treated with AGEs (400 μg/mL) alone, melatonin (50 μM) + AGEs (400 μg/mL), or AGEs (400 μg/mL) + melatonin (50 μM) combined with chloroquine (CQ; 10 μM). a, b Mitochondrial membrane potential was detected using MitoTracker Red CMXRos, and nuclei were stained with Hoechst. Scale bar, 25 μm. c,e Protein expression levels of LC3, p62, and GAPDH in EPCs of each group treated as described above. f, h Protein expression levels of Bcl-2, Bax, cytochrome c, caspase-9, cleaved caspase-3, and GAPDH in EPCs of each group treated as described above. Data are presented as the mean ± SEM. Significant differences between the treatment and control groups are indicated as **P < 0.01 or *P < 0.05. n = 3