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. 2018 Nov 21;50(11):154. doi: 10.1038/s12276-018-0177-z

Fig. 5. Melatonin promotes autophagy flux by activating the AMPK/mTOR pathway in AGE-treated EPCs.

Fig. 5

EPCs were pre-treated with 50 μM melatonin and 50 μM Compound C (Cpd C) for 2 h. Next, 400 μg/mL AGEs were added for an additional 6 h. a, c Representative Western blots and quantification data of p-AMPK/AMPK, p-mTOR/mTOR, p-p70s6k/p70s6k, and p-4EBP1/4EBP1 of each group treated with AGEs, melatonin, and Cpd C. b, d Representative Western blots and quantification data of LC3 and P62 of each group treated with AGEs, melatonin and Cpd C. Data are presented as the mean ± SEM. Significant differences between the treatment and control groups are indicated as **P < 0.01 or *P < 0.05. n = 3