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Binding of COMP to the C-terminal motif of collagen II contained in peptide 52. Peptide 52 of the collagen II foldon library was coated to an ELISA plate and incubated with COMP protein. Binding was detected with a specific anti-COMP antibody. Bars indicate standard deviation of triplicates. The peptide sequence is depicted in Table 1. The fitted KD is 4.1 ± 0.8 nM.
