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. 2018 Nov 21;8:17187. doi: 10.1038/s41598-018-35447-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Binding of TSP4 to the GVKGHR motif. The minimal binding site GVKGHR expressed as a foldon construct was coated to an ELISA plate and incubated with TSP4. Binding was detected with a specific anti-TSP4 antibody. Bars indicate standard deviation of triplicates. The fitted K_D is 0.56 ± 0.05 nM.