Table 2.

Peptides used for further analysis of the minimal binding site.

Sequence	Apparent KD (nM)	Melting point (°C)
GARGFPGTPGLPGVKGHRGYPGLDGA	19.3 ± 12.4	36.1 ± 0.5
GLPGVKGHR	n.d.	48.2 ± 0.6
GPPGVKGHR	3.57 ± 0.37	54.2 ± 0.7
GLPGPKGHR	2.26 ± 0.39	52.5 ± 0.6
GLPGVPGHR	n.a.	51.1 ± 0.3
GLPGVKGPR	496 ± 3203	n.d.
GLPGVKGHP	n.a.	47.9 ± 0.4
GVKGHR	1.61 ± 0.29	56.5 ± 0.6

The sequence unique to peptide 5 is underlined and mutations in comparison to the wt sequence are marked in bold. All peptide sequences are presented in an N-Strep-(GPP)₆-GUEST-(GPP)₆-foldon context. Melting points were determined by CD spectroscopy at 210 nm. Exemplary spectra can be found in the Fig. S1.

(n.d. - not determined; n.a. - not applicable/no measurable signals observed to fit K_D values).