FIGURE 3.

¹H NMR profiles of herbal extracts from the 15 spleen-meridian herbs. The extracts (50 mg) were dissolved in 400 μL of D₂O and centrifuged at 13,000 × g for 1 min before transferred to a standard 5-mm NMR tube. The ¹H NMR experiment were conducted on a Varian 300 MHz NMR spectrometer, operating at 300.13 MHz ¹H NMR frequency at 298 K. The spectra were acquired using a 1D CPMG pulse sequence (RD-90°-t1-90°-tm- 90°-acquire) to generate a spectrum with a reduced residual solvent peak. The range of chemical shift was set from –2 to 12 ppm, and the deuterated solvent peak at 4.8 was set as calibration. The extracting solvents are indicated. The extracts from 3 batches of herbs were subjected to analysis, a representative profile was shown. a, Codonopsis Radix; b, Atractylodis Macrocephalae Rhizoma; c, Angelicae Sinensis Radix; d, Astragali Radix; e, Jujubae Fructus; f, Ginseng Radix; g, Amomi Fructus; h, Aucklandiae Radix; i, Areca Catechu; j, Dioscoreae Ahizoma; k, Lablab Semen Album; l, Poria Cocos; m, Nelumbinis Semen; n, Coicis Semen; o, Crataegi Fructus.