Table 3.
The correlation between anti-oxidant profiles of the spleen-meridian herbs with the “Yin-Yang” classification criterion.
| AttributesHerbs | Total phenolics (mg GAE/g)b | DPPH radical scavenging EC50 (mg/mL) | tBHP protection(%) | tBHP protection EC50 (mg/mL) | ROS formation (% of inhibition) | pARE-luciferase activity (% of increase) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Wc | E50 | E90 | E90 | E50 | E90 | W | E50 | E90 | W | E50 | E90 | W | E50 | E90 | W | E50 | E90 | ||
| Yang herbs | CRa | 10.21 ± 0.32d |
12.25 ± 0.22 |
9.71 ± 0.21 |
0.82 ± 0.03∗ |
1.44 ± 0.04 |
1.39 ± 0.05 |
32.7 ± 0.97 |
51.8 ± 2.34∗∗ |
23.6 ± 0.92 |
0.72 ± 0.05 |
0.88 ± 0.1 |
0.8 ± 0.1 |
29.72 ± 1.22 |
38.37 ± 1.41∗ |
17.41 ± 1.18 |
21.82 ± 1.72 |
40.94 ± 4.09∗ |
18.21 ± 2.82 |
| AMR | 17.91 ± 0.32∗ |
20.31 ± 0.24∗∗ |
16.19 ± 0.23 |
0.46 ± 0.01∗∗ |
0.64 ± 0.02∗ |
0.55 ± 0.02∗∗ |
53 ± 1.65∗∗ |
61.3 ± 0.67∗∗∗ |
42.8 ± 2.53 |
0.05 ± 0.01∗∗∗ |
0.2 ± 0∗∗∗ |
0.18 ± 0∗∗∗ |
35.28 ± 1.39∗ |
50.36 ± 1.89∗∗ |
38.14 ± 1.3∗ |
38.68 ± 4.19∗ |
49.04 ± 6.19∗∗ |
29.82 ± 3.8 |
|
| ASR | 17.61 ± 0.22∗ |
19.5 ± 0.26∗∗ |
13.25 ± 0.11 |
0.47 ± 0.02∗∗ |
0.72 ± 0.02∗ |
0.62 ± 0.03∗ |
58.4 ± 1.21∗∗∗ |
73.1 ± 1.63∗∗∗ |
53.6 ± 1.71∗∗ |
0.06 ± 0.01∗∗∗ |
0.1 ± 0∗∗∗ |
0.03 ± 0.01∗∗∗ |
45.01 ± 1.46∗∗ |
53.61 ± 1.57∗∗ |
31.89 ± 1.33 |
45.75 ± 3.15∗∗ |
57.02 ± 2.57∗∗ |
32.62 ± 3.26 |
|
| AsR | 9.02 ± 0.21 |
10.93 ± 0.31 |
8.92 ± 0.21 |
1.36 ± 0.02 |
1.52 ± 0.04 |
1.5 ± 0.06 |
62.5 ± 1.87∗∗∗ |
38.7 ± 2.39 |
37.6 ± 0.82 |
0.8 ± 0.01 |
0.19 ± 0∗∗ |
0.67 ± 0 |
41.72 ± 1.44∗ |
32.46 ± 1.33 |
29.63 ± 1.29 |
43.72 ± 2.37∗ |
33.48 ± 1.85 |
28.86 ± 1.89 |
|
| JF | 8.45 ± 0.31 |
6.18 ± 0.11 |
7.02 ± 0.21 |
2.19 ± 0.08 |
1.75 ± 0.03 |
1.53 ± 0.06 |
42.5 ± 1.93 |
57.7 ± 1.92∗∗∗ |
47.8 ± 1.28∗ |
0.3 ± 0.01∗ |
0.75 ± 0 |
0.74 ± 0.01 |
36.12 ± 1.3 |
56.49 ± 1.75∗∗ |
33.37 ± 1.48 |
30.33 ± 3.03 |
54.54 ± 4.54∗∗ |
47.64 ± 2.48∗∗ |
|
| GR | 17.21 ± 0.33∗ |
24.91 ± 0.43∗∗ |
16.23 ± 0.43 |
1.17 ± 0.03 |
1.36 ± 0.02 |
1.94 ± 0.03 |
57.7 ± 1.76∗∗∗ |
63.3 ± 1.45∗∗∗ |
75.6 ± 1.13∗∗∗ |
0.04 ± 0.01∗∗∗ |
0.04 ± 0.01∗∗∗ |
0.04 ± 0.01∗∗∗ |
12.47 ± 1.12 |
19.44 ± 1.2 |
47.46 ± 1.47∗∗ |
21.67 ± 1.22 |
19.82 ± 1.4 |
46.81 ± 1.57∗∗ |
|
| AF | 1.11 ± 0.02 |
4.17 ± 0.03 |
7.89 ± 0.07 |
2.41 ± 0.05 |
1.66 ± 0.05 |
2.83 ± 0.06 |
38.4 ± 1.08 |
40.7 ± 0.57 |
29.6 ± 0.81 |
0.34 ± 0 |
0.36 ± 0.01 |
0.83 ± 0 |
24.16 ± 1.27 |
34.03 ± 1.29 |
28.13 ± 1.26 |
27.47 ± 0.87 |
29.38 ± 6.29 |
26.28 ± 5.55 |
|
| AuR | 5.94 ± 0.23 |
11.06 ± 0.36 |
5.03 ± 0.27 |
1.19 ± 0.05 |
2.27 ± 0.07 |
2.98 ± 0.07 |
8.5 ± 1.45 |
16.3 ± 1.91 |
15.2 ± 1.91 |
0.1 ± 0∗∗∗ |
0.1 ± 0∗∗∗ |
0.1 ± 0∗∗∗ |
14.56 ± 1.14 |
18.55 ± 1.17 |
23.23 ± 1.22 |
5.63 ± 3.63 |
13.63 ± 3.98 |
17.4 ± 0.67 |
|
| AC | 19.28 ± 0.32∗∗ |
21.73 ± 0.34∗∗ |
35.97 ± 0.46∗∗∗ |
0.39 ± 0.01∗∗ |
0.22 ± 0.01∗∗ |
0.48 ± 0.02∗∗ |
11.1 ± 0.27 |
53.4 ± 1.85∗∗ |
56.3 ± 0.36∗∗ |
0.09 ± 0∗∗∗ |
0.08 ± 0∗∗∗ |
0.08 ± 0∗∗∗ |
9.88 ± 1.1 |
34.71 ± 1.34 |
39.94 ± 1.39∗ |
9.59 ± 3.96 |
34.4 ± 3.44 |
39.17 ± 2 |
|
| Neutral herbs | DA | 12.79 ± 0.44 |
15.1 ± 0.54 |
12.45 ± 0.52 |
1.24 ± 0.01 |
1.34 ± 0.02 |
1.23 ± 0.01 |
14 ± 1.65 |
24.2 ± 0.51 |
21.4 ± 1.44 |
4.43 ± 0.07 |
5.04 ± 0.06 |
6.5 ± 0.06 |
19.26 ± 1.12 |
16.84 ± 1.19 |
19.06 ± 1.14 |
11.74 ± 1.12 |
19.06 ± 1.91 |
14.13 ± 1.41 |
| LSA | 0.14 ± 0.01 |
0.72 ± 0.02 |
1.14 ± 0.03 |
3.39 ± 0.09 |
3.17 ± 0.05 |
2.74 ± 0.09 |
24.4 ± 2.05 |
27.5 ± 0.92 |
25 ± 1.94 |
0.09 ± 0∗∗∗ |
0.09 ± 0∗∗∗ |
0.09 ± 0∗∗∗ |
16.32 ± 1.11 |
20.56 ± 1.3 |
18.65 ± 1.18 |
11.42 ± 1.01 |
29.54 ± 0.9 |
17.73 ± 0.77 |
|
| PC | 12.57 ± 0.54 |
16.84 ± 0.51 |
13.31 ± 0.41 |
0.95 ± 0.01∗ |
0.74 ± 0.01∗ |
0.99 ± 0.01∗ |
43.7 ± 1.52 |
53.4 ± 2.2∗∗ |
48.6 ± 0.85∗ |
0.04 ± 0.01∗∗∗ |
0.04 ± 0.01∗∗∗ |
0.04 ± 0∗∗∗ |
40.81 ± 1.42∗ |
46.67 ± 1.43∗∗ |
47.86 ± 1.5∗∗ |
41.88 ± 0.82∗ |
43.03 ± 1.93∗ |
49.92 ± 0.8∗∗ |
|
| Yin herbs | NS | 6.97 ± 0.32 |
12.09 ± 0.34 |
6.58 ± 0.42 |
1.11 ± 0.06 |
2.12 ± 0.09 |
1.98 ± 0.08 |
26.2 ± 1.92 |
12.6 ± 0.71 |
25.2 ± 1.8 |
0.14 ± 0.05∗∗∗ |
0.14 ± 0.04∗∗∗ |
0.13 ± 0.04∗∗∗ |
24.26 ± 1.21 |
16.54 ± 1.1 |
23.84 ± 1.25 |
21.03 ± 0.81 |
10.02 ± 1.1 |
24.88 ± 2.49 |
| CS | 12.07 ± 0.44 |
7.47 ± 0.32 |
9.9 ± 0.37 |
1.72 ± 0.02 |
1.41 ± 0.01 |
1.19 ± 0.01 |
29.7 ± 1.33 |
20.8 ± 2.08 |
21.4 ± 1.47 |
0.49 ± 0.03 |
0.21 ± 0.04∗∗∗ |
0.21 ± 0.04∗∗∗ |
26.31 ± 1.16 |
20.06 ± 1.17 |
21.73 ± 1.21 |
15.7 ± 1.16 |
17.18 ± 3.72 |
21.37 ± 1.53 |
|
| CF | 39.72 ± 0.11∗∗∗ |
19.14 ± 0.17∗∗ |
21.94 ± 0.21∗∗ |
0.48 ± 0.08∗∗ |
0.34 ± 0.08∗∗ |
0.26 ± 0.05∗∗ |
25.8 ± 2.25 |
26.5 ± 1.89 |
33.4 ± 1.44 |
0.32 ± 0.02∗∗ |
1.65 ± 0.04 |
0.88 ± 0.03 |
44.91 ± 1.49∗∗ |
34.68 ± 1.19 |
37.36 ± 1.14∗ |
39.12 ± 4.91∗ |
38.57 ± 1.86∗ |
44.06 ± 4.04∗ |
|
aCR, Codonopsis Radix; AMR, Atractylodis Macrocephalae Rhizoma; ASR, Angelicae Sinensis Radix; AsR, Astragali Radix; JF, Jujubae Fructus; GR, Ginseng Radix; AF, Amomi Fructus; AuR, Aucklandiae Radix; AC, Areca Catechu; DA, Dioscoreae Ahizoma; LSA, Lablab Semen Album; PC, Poria Cocos; NS, Nelumbinis Semen; CS, Coicis Semen; CF, Crataegi Fructus. bGAE, gallic acid equivalent. cW, water extract; E50, 50% ethanol extract; E90, 90% ethanol extract. dValues are expressed as Mean ± SD, where n = 4, each with triplicate samples. Statistical comparison was made with mean value (mean value of total phenolics is 12.87 mg GAE/g; mean value of DPPH radical scavenging EC50 is 1.38 mg/mL; mean value of tBHP protection effect is 37.9%; mean value of tBHP protection effect EC50 is 0.65 mg/mL; mean value of inhibition effects to ROS formation is 30.5%; mean value of pARE-luciferase activity increase is 29.6%). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.