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. 2018 Nov 21;8:17179. doi: 10.1038/s41598-018-35615-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Characterization of standard ligands at the hH₁R, the hM_1,3,5R and the hNTS₁R. Live HEK293T cells, stably expressing the developed sensor and the indicated receptor, were analysed regarding their response to standard agonists (A) and antagonists (B) for the respective receptors. The substrate D-luciferin was added directly to the cells, and the experiment was carried out at 37 °C. Agonist data was normalized to a reference full agonist for each receptor, maximal stimulation of which was defined as 100% (hH₁R: histamine, hM_1,5R: carbachol, hM₃R: oxotremorine). pEC₅₀, E_max and pK_b values are listed in Table 1 and were in good accordance with data described in literature. Data are presented as means ± SEM of at least three independent experiments, each performed in triplicate. s.c.: solvent control.