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. 2018 Nov 21;8:17195. doi: 10.1038/s41598-018-35479-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Screening of genome-edited cells by Two-Step ORNi-PCR. (A) Schematic diagram of the screening of genome-edited cells by Two-Step ORNi-PCR. (B) Target position of genome editing and sequence of ORN_FOS. The forward DNA sequence of the allele is shown. The target sites of TALEN-FOS-left and -right are underlined. Nucleotides in the spacer region surrounded by TALEN-FOS-left and -right are cleaved. (C) Results of Two-Step ORNi-PCR. The experimental conditions shown in Fig. 5E were used for Two-Step ORNi-PCR. M, molecular weight markers. (D) Schematic diagram of the confirmation of indel mutations. (E) Types of indel mutation. N.C., not confirmed.