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. 2018 Nov 21;8:17174. doi: 10.1038/s41598-018-33873-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Surface NCL contains more 9-O-Ac-Sia than cytoplasmic 100 kDa NCL. (a) Quantitative comparison of 9-O-Ac-Sia-containing NCL immunoprecipitated from an equal cell equivalent of cell surface and intracellular fractions. (b) Western blot analysis using the detection reagents indicated to the left on total cell lysates, cell surface proteins isolated by live cell biotinylation and streptavidin-column isolation and their intracellular fractions. Note: equal amounts of protein lysate were loaded in each lane. Blots were sequentially stripped and re-probed.