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. 2018 Nov 21;9(12):1158. doi: 10.1038/s41419-018-1170-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b Luciferase reporter plasmids containing WT or mutant ZEB 3′UTR were co-transfected into BGC-823 and SGC-7901 cells with miR-203 mimics in parallel with Ctrl mimics. Each experiment was repeated three times. Data are expressed as the mean ± SD. *p < 0.05 and **p < 0.01. c, d RIP with mouse monoclonal anti-Ago2, IgG, or 10% input from SGC-7901 and BGC-823 cell extracts. UCA1 and miR-203 expression levels in immunoprecipitates were determined by qRT-PCR. Numbers are mean ± SD. (n = 3). **p < 0.01 and ***p < 0.001. e PCR products of UCA1 and miR-203 were detected by gel electrophoresis. f, g q-PCR analysis of ZEB2 expression level in SGC-7901 and BGC-823 cells transfected with different doses of miR-203 mimics and control. The error bars in graphs represented SD, and each experiment was repeated three times. *p < 0.05. h, i q-PCR analysis of ZEB2 expression level in SGC-7901 and BGC-823 stable cells transfected with different doses of miR-203 mimics and control. The error bars in graphs represented SD, and each experiment was repeated three times. *p < 0.05. j Western-blot analysis of ZEB2 in BGC-823 and SGC-7901 cells transfected with Lv-control or Lv-UCA1, Lv-UCA1-Mut, and Lv-UCA1 along with miR-203 mimics (Left panel). Western blot to analyze ZEB2 expression level in BGC-823 and SGC-7901 cells transfected with UCA1 siRNA or control along with miR-203 inhibitor (Right panel)