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mCD300c2 suppresses cytokine production from T cells. Purified murine T cells were cultured with plate-bound anti-CD3 antibody (1 μg/ml) in the presence of graded doses of mCD300c2-Ig protein or control Ig protein for 3 days as Figure 3A. The levels of IFNγ, TNFα, IL-2, IL-17A, and IL-10 in the supernatant were measured by ELISA kits. The data were pooled from 3 independent experiments and expressed as mean ± SD. *P < 0.05 compared with control Ig.
