Fig. 4.

The location of OsNRAMP2 on chromosome 3 and sequence differences and expression analysis of OsNRAMP2. a, Colocalization of OsNRAMP2 with qCd3–2. The red arrow indicates the location and direction of OsNRAMP2. b, Sequence differences of OsNRAMP2 protein and the respective causal nucleotide differences between high and low Cd accumulation accessions. Sequence differences between high and low Cd accumulation rice accessions are indicated with different background colors. The numbers on the left of each local alignment indicate the position of the first nucleotide/amino acid for the alignment. c, Expression changes of OsNRAMP2 in roots after Cd treatment between high and low Cd accumulation rice accessions. d, Expression changes of OsNRAMP2 in shoot after Cd treatment between high and low Cd accumulation rice accessions