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fU-Seq, a Method that Utilizes Selective Tagging of 5fU with an Azido-Modified Reagent
(A) The chemical structures of 5fU, the azido-modified reagent, DBCO derivatives, and their products.
(B) Schematic illustration of fU-Seq. Genomic DNA is fragmented, sequentially labeled with azi-BIAN, conjugated to biotin for pull-down enrichment, and released following enrichment using DTT to define 5fU sites throughout the genome. NGS, next-generation sequencing.
