Figure 4.
p62 Interacts with USP10
(A) Cell lysates prepared from control (NT) and USP10-KD (USP10-1) HeLa cells were immunoprecipitated with the anti-p62 antibody or normal rabbit IgG. The cell lysate (Input) and immunoprecipitates (IP) were characterized by western blot (WB) with anti-USP10, anti-HDAC6, anti-dynein intermediate chain (IC), and anti-p62 antibodies.
(B) HeLa cells were transfected with the GFP-CFTR-ΔF508 plasmid or empty control. Cell lysates (Input) and immunoprecipitates with the anti-GFP antibody (IP) were characterized by WB using anti-USP10 and anti-GFP antibodies. The asterisk indicates a nonspecific band.
(C–E) HeLa cells were transfected with HA-USP10 or its mutant (USP101−274, USP101−214, USP101−154, or USP101−149 in C; USP1096−798, USP10215−798, or USP10275−798 in D; and USP10410−798 or USP10594−798 in E) plasmid. Cell lysates (Input) and immunoprecipitates with the anti-p62 antibody (IP) were characterized by WB with anti-HA and anti-p62 antibodies.