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. 2018 Nov 15;11:415. doi: 10.3389/fnmol.2018.00415

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Copyright © 2018 Suh, Zhou, Fernandez-Carasa, Clemenson, Pons-Espinal, Ro, Marti, Raya, Gage and Consiglio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Long-term maintenance of NSCs in the adult hippocampus. Fate mapping of GFP⁺ identified NSCs that proliferate and produce neurons (A) and astrocytes (B). Some NSCs underwent cell proliferation proliferated twice in a one-month interval (C). GFP-labeled cells in vivo gave rise to in vitro NSCs. In vitro, GFP⁺ NSCs expressed NSC markers such as NESTIN and Sox2 (D) and differentiated into neurons (TUJ1) and astrocytes (GFAP) (E). GFP⁺ derived-neurons and astrocytes at day 7 of differentiation (F).