Figure 1.

OVA-specific CTL become activated in cMy-mOVA mice after TAC. The cytotoxic activity of splenocytes against RMA-OVA and RMA-con target cells was determined by ⁵¹Cr-release assays at several CD3⁺CD8⁺ effector cell to target cell (E:T) ratios 10 weeks after (A) sham (n = 13) or (B) TAC (n = 9) surgery. Means and standard errors of the mean (SEM) are displayed. Differences between sham and TAC-operated mice were analyzed by 2-way ANOVA adjusted for the E:T ratios and the respective P-values are indicated. (C) The proportion of mice exerting a higher cytotoxic activity against RMA-OVA than RMA-con cells is displayed. The difference between sham and TAC-operated mice has been analyzed by Fisher‘s exact test and the P-value is displayed. (D) The OVA-specific cellular cytotoxicity was higher in some mice after TAC. Means and SEM of triplets are shown for mouse 112. (E) The lack of an OVA-specific cytotoxicity in mouse 109 that also underwent TAC is shown for comparison.