Figure 3.

More infiltration of CD3⁺ and CD8⁺ cells in the myocardium of cMy-mOVA+OT-I than cMy-mOVA mice. (A) The infiltration of CD3⁺ and CD8⁺ cells was analyzed by immunohistochemistry in the myocardium of sham and TAC-operated cMy-mOVA and cMy-mOVA+OT-I as illustrated here. The bars indicate 50 μm. The arrow heads point to CD3⁺ or CD8⁺ cells, respectively. The numbers of infiltrating CD3⁺-T cells (B), CD8⁺ cells (C) CD4⁺ cells (D), CD45R(B220)⁺ B cells (E) and F4/80⁺ monocytes/macrophages (F) were determined in the myocardium after 10 weeks in sham and TAC-operated cMy-mOVA (sham n = 14, TAC n = 10) and cMy-mOVA+OT-I mice (sham n = 11, TAC n = 9) and means plus SEM are shown. The P-value of a Kruskal-Wallis test comparing all groups is indicated. Differences between two groups were analyzed by U-tests and significant P-values are given in the panels (^*P < 0.05, ^**P < 0.01, ^***P < 0.001). The Bonferroni-Holm correction was used to adjust for multiple testing in the two group comparisons.