Figure 5.

OVA-specific CTL become activated in cMy-mOVA+OT-I mice after TAC and have a higher cytotoxic activity than OVA-specific CTL in cMy-mOVA mice. (A) The cytotoxic activity of splenocytes against RMA-OVA and RMA-con target cells was determined by ⁵¹Cr-release assays at several CD3⁺CD8⁺ E:T ratios 10 weeks after sham (n = 10) or TAC (n = 9) surgery either directly after autopsy (day 0) or after restimulation in vitro with 1 μM OVA (day 4). Means and SEM are displayed. Differences between sham and TAC-operated mice were analyzed by 2-way ANOVA adjusted for the E:T ratios and the respective P-values are indicated (ns, non-significant). (B) The proportion of mice exerting a higher cytotoxic activity against RMA-OVA than RMA-con cells is displayed. The difference between sham and TAC-operated mice has been analyzed by Fisher‘s exact test and the P-value is displayed. (C) The OVA-specific cytotoxicity against RMA-OVA target cells of CTL from cMy-mOVA and cMy-mOVA+OT-I mice after sham (n = 9) or TAC (n = 9) surgery is compared by 2-way ANOVA adjusted for the E:T ratios and the respective P-value is indicated.