Table 1.
Characteristics of ten VSCC with metastatic regional lymph nodes, distant metastases or recurrent lesions included in the study. LN= lymph node. Histology classification (histopathologic type of squamous cell carcinoma: basaloid, mixed, keratinizing) performed by pathologist (MK). Stage at diagnosis. Viral load = copies of HPV16 E6 in 20 ng of total DNA. Viral load per cell = viral copies/(HBB copies/2). E1/E6 and E2/E6 = ratios of viral copy numbers of E1 and E2 compared to E6. E2BS3 and 4 = mean of methylation levels in E6 positions 37, 43, 52 and 58. Distant met. = distant metastasis.
|
Patients |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | ||
| Primary VSCC | Stage IV | Stage III | Stage III | Stage IV | Stage II | Stage II | Stage IV | Stage II | Stage III | Stage II | |
| Mixed | Basaloid | Keratinizing | Keratinizing | Mixed | Basaloid | Basaloid | Mixed | Basaloid | Basaloid | ||
| Regional LN | At diagnosis | At diagnosis | At diagnosis | After disease free interval | At diagnosis | After disease free interval | At diagnosis | ||||
| Distant met. | Lymphoid | Hematogenous | |||||||||
| First Recurrence | Mixed | Basaloid | Keratinizing | Mixed | Basaloid | ||||||
| Last Recurrence | Basaloid | ||||||||||
| HPV type | Primary VSCC | 16 | 16 | 16 | 16 | 16 | 16 | 16 | 16 | 16 | 16 |
| Regional LN | 16 | 16 | 16 | 16 | 16 | 16 | 16 | ||||
| Distant met. | 16 | 16 | |||||||||
| Recurrence | 16 | 16 | 16 | 16 | 16 | ||||||
| Recurrence | 16 | ||||||||||
| HPV16 variant | Primary VSCC | E-p | E-G350G | E-p | E-C109G | AA/NA1 | E-p | E-G131T | E-p | E-G350G | E-G350G |
| Regional LN | E-p | E-G350G | E-p | E-C109G | E-G131T | E-p | E-G350G | ||||
| Distant met. | E-G350G | E-G350G | |||||||||
| Recurrence | AA/NA1 | E-p | E-G131T | E-p | E-G350G | ||||||
| Recurrence | E-p | ||||||||||
| Viral load | Primary VSCC | 5396 | 59.3 | 21.1 | 2236 | 573 | 16.1 | 2637 | 11520 | 8.47 | 17010 |
| Regional LN | 1283 | 16.1 | 40.7 | 116 | 1318 | 8960 | 0.79 | ||||
| Distant met. | 17 | 2488 | |||||||||
| Recurrence | 674 | 23 | 981 | 28270 | 16550 | ||||||
| Recurrence | 16.2 | ||||||||||
| Viral load per cell | Primary VSCC | 105.8 | 1.85 | 5.17 | 62.3 | 32.1 | 0.59 | 320 | 1477 | 0.275 | 565 |
| Regional LN | 22.7 | 7.16 | 20.6 | 245 | 31.9 | 586 | 0.003 | ||||
| Distant met. | 0.33 | 363 | |||||||||
| Recurrence | 27.7 | 2.5 | 50 | 1303 | 470 | ||||||
| Recurrence | 1.71 | ||||||||||
| E1/E6 | Primary VSCC | 4.78 | 0.54 | 0.74 | 0.68 | 0.03 | 0.78 | 0.65 | 0.73 | 0.87 | 1.04 |
| Regional LN | 4.29 | 0.52 | 0.61 | 0.51 | 0.75 | 0.65 | 0.46 | ||||
| Distant met. | 0.90 | 0.85 | |||||||||
| Recurrence | 0.11 | 0.74 | 0.64 | 0.72 | 0.89 | ||||||
| Recurrence | 0.73 | ||||||||||
| E2/E6 | Primary VSCC | 6.34 | No result | 4.29 | 1.14 | 0.82 | 0.02 | 0.92 | 0.05 | 1.37 | 2.46 |
| Regional LN | 9.10 | No result | 2.22 | 1.08 | 1.09 | 0.05 | 0.98 | ||||
| Distant met. | 1.78 | 1.69 | |||||||||
| Recurrence | 1.13 | No result | 0.84 | 0.03 | 1.19 | ||||||
| Recurrence | No result | ||||||||||
| E2BS3 and 4 methylation (%) | Primary VSCC | 4.7 | 3.2 | 3.6 | 2.0 | 1.6 | 3.0 | 10 | 91.6 | No result | 73.9 |
| Regional LN | 4.9 | 2.3 | 2.5 | 1.1 | 12 | 90.4 | 1.5 | ||||
| Distant met. | No result | 55.1 | |||||||||
| Recurrence | 2.1 | 1.5 | 5.9 | 86.2 | 50.6 | ||||||
| Recurrence | 2.6 | ||||||||||