Table 3.
Morphological analyses of the dendritic arboures of hippocampal neurones uncovers deficits in P60 pallid Dentate Gyrus granule cells, suggesting that lack of BLOC-1 negatively affects neurite outgrowth also in vivo.
| Two-way ANOVA | ||||
|---|---|---|---|---|
| Genotype | Distance from the soma | Interaction | ||
| C | F(1) = 26.9; P < 0.0001 | F(10) = 145; P < 0.0001 | F(10) = 3.13; P = 0.0015 | |
| D | F(1) = 2.08; P = 0.1535 | F(9) = 14.0; P < 0.0001 | F(9) = 0.08; P = 0.9998 | |
| E | F(1) = 0.02; P = 0.8998 | F(10) = 240; P < 0.0001 | F(10) = 0.06; P = 1.0000 | |
| Number of dendrites | WT | Pallid | One-way ANOVA | |
| DG | Primary | 2.82 ± 0.14 | 2.12 ± 0.08∗∗ | F(5) = 97.14; P < 0.0001 |
| Secondary | 4.97 ± 0.16 | 3.92 ± 0.08∗∗∗∗ | ||
| Tertiary | 5.96 ± 0.20 | 4.83 ± 0.16∗∗∗∗ | ||
| CA1 Apical | Secondary | 6.47 ± 0.64 | 5.91 ± 0.87 | F(3) = 17.94; P < 0.0001 |
| Tertiary | 2.04 ± 0.23 | 1.99 ± 0.30 | ||
| CA1 Basal | Primary | 4.43 ± 0.12 | 4.25 ± 0.07 | F(5) = 32.18; P < 0.0001 |
| Secondary | 6.81 ± 0.26 | 7.07 ± 0.34 | ||
| Tertiary | 7.29 ± 0.38 | 7.33 ± 0.20 | ||
| Total branching points | Student’s t-test | |||
| DG | 7.99 ± 0.09 | 6.80 ± 0.39∗∗ | t(10) = 2.97; P = 0.0141 | |
| CA1 | Apical | 7.59 ± 0.57 | 6.79 ± 0.92 | t(8) = 0.74; P = 0.4829 |
| Basal | 11.5 ± 0.29 | 10.9 ± 0.61 | t(8) = 0.84; P = 0.4269 | |
| Length of dendrites | One-way ANOVA | |||
| DG | Primary | 31.81 ± 1.47 | 32.64 ± 1.50 | F(5) = 117.1; P < 0.0001 |
| Secondary | 58.37 ± 2.63 | 64.80 ± 1.62 | ||
| Tertiary | 75.27 ± 1.34 | 76.24 ± 2.16 | ||
| CA1 Apical | Primary | 109.8 ± 5.20 | 120.6 ± 10.5 | F(5) = 27.56; P < 0.0001 |
| Secondary | 63.82 ± 2.04 | 62.91 ± 2.02 | ||
| Tertiary | 50.25 ± 4.95 | 58.12 ± 4.68 | ||
| CA1 Basal | Primary | 29.97 ± 2.85 | 31.14 ± 1.47 | F(5) = 26.51; P < 0.0001 |
| Secondary | 41.99 ± 0.75 | 48.91 ± 0.59 | ||
| Tertiary | 52.62 ± 4.00 | 56.25 ± 0.62 | ||
Two-way ANOVA followed by Bonferroni’s Multiple Comparison Test was used to evaluate the impact of genotype and distance on the number of processes of DG granule cells and CA1 pyramidal neurones crossing fixed-distance concentric circles (n = 5–6 animals/genotype), and revealed significant effects of both variables in the DG. A significant decrease in the number of dendrites and branching points was observed in pallid DG granule cells, with no differences in their length. Data are shown as the mean ± SEM of 12–15 neurones per animal/6 animals/region/genotype; One-way ANOVA followed by Bonferroni’s Multiple Comparison test: ∗∗P < 0.01, ∗∗∗∗P < 0.0001; differences in total branching points between genotypes were assessed by Student’s t-test. Degrees of freedom are reported in parentheses. Bold values indicate statistically significant genotypic differences. Alpha = 0.05, WT, wild-type.