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. 2018 Nov 15;9:1601. doi: 10.3389/fphys.2018.01601

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Copyright © 2018 Ma, Zhang, Liu, Chen, Xu, Xiao and Liang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockdown of HaMet in 5th-instar H. armigera larvae. (A) The induction of HaMet expression by Methoprene (JH analog) or 20E. (B) The expression levels of HaMet in larval midgut after dsGFP or dsHaMet treatment examined at 24, 48, 72, and 96 h after treatment. (C) Statistical analysis of the pupation time and pupation rate after HaMet knockdown (30 larvae, three replicates). (D) The abnormal pupation after HaMet knockdown. Error bars indicate SE, ^∗denotes P < 0.05, ^∗∗denotes P < 0.01 (Student’s t-test). Different letters indicate significant difference between specimens (P < 0.05). ns, indicates no significant difference (P > 0.05).