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. 2018 Nov 15;9:2727. doi: 10.3389/fmicb.2018.02727

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Copyright © 2018 Yang, Zeng, Wang, Cheng, Pan, Zhu, Liu, Jia, Yang, Wu, Chen, Zhao, Zhang, Liu, Yu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression level and RNA level of pcDNA3.1-RFP-2A1-EGFP. Experimental materials of each panel were from the same batch of samples. (A) The protein expression of pcDNA3.1-RFP-2A1-EGFP. The top half was immunoblotted with EGFP mAb. The bottom half was immunoblotted with RFP mAb. EGFP and RFP represent the control plasmid pEGFP-N1 and pDsRed-Express-C1, respectively. uTm represent the sample which was not transfected. (B) Expression comparison of upstream RFP and downstream EGFP in pcDNA3.1-RFP-2A1-EGFP. (C) The transcriptional level of pcDNA3.1-RFP-2A1-EGFP. Five biological duplication were performed in each panels. Statistically significant differences were determined by one-way ANOVA. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001, ^∗∗∗∗P < 0.0001 indicate the level of statistical significance of differences between different groups.