Table 2.
The primers used to mutate the critical amino acids.
| G14 | G-A | F: TCACTACTGAAGCAGTGGAACCGAA |
| R: TTCGGTTCCACTGCTTCAGTAGTGA | ||
| E16 | E-A | F: CTGAAGGAGTGGCACCGAACCCAGG |
| R: CCTGGGTTCGGTGCCACTCCTTCAG | ||
| N18 | N-N’ | F1:GGAGTGGAACCGGACCCAGGACCTA |
| R1: TAGGTCCTGGGTCCGGTTCCACTCC | ||
| N18 | N’-A | F2: GAGTGGAACCGGCCCCAGGACCTAT |
| R2: ATAGGTCCTGGGGCCGGTTCCACTC | ||
| P19 | P-A | F: GTGGAACCGAACGCAGGACCTATGG |
| R: CCATAGGTCCTGCGTTCGGTTCCAC | ||
| G20 | G-A | F: AACCGAACCCAGCACCTATGGCCTC |
| R: GAGGCCATAGGTGCTGGGTTCGGTT | ||
| P21 | P-A | F: CCGAACCCAGGAGCTATGGCCTCCT |
| R: AGGAGGCCATAGCTCCTGGGTTCGG |