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. 2018 Nov 13;12:822. doi: 10.3389/fnins.2018.00822

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Copyright © 2018 Meas, Nishimura, Scheibinger and Dabdoub.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Whole organ explant culture of the spiral ganglion. (A–C) Spiral ganglion explants cultured on 2% Matrigel-coated dishes for 1 day (A), 2 days (B), or 3 days (C). Neurons are stained with TuJ1 (red) and glial cells are stained with Sox2 (green) antibodies. Scale bar;100 μm (D,E) Magnified images from spiral ganglion explants cultured for 3 days. Neurons are amongst glial cells. Scale bar; 10 μm.