Figure 3.

Infectious virus titers and RNA in blood and tissue specimens from guinea pig–adapted Sudan virus (GA-SUDV)–infected guinea pigs. A, Whole blood and tissue specimens were collected 9 days after infection from GA-SUDV–infected guinea pigs (n = 3 for each treatment group) treated with control or with 150 mg/kg/day or 300 mg/kg/day of T-705, starting 2 days after infection and continuing every day up to day 8. Infectious virus titers in blood specimens and tissue homogenates of liver, spleen, kidney, and lungs were determined by a 50% tissue culture infective dose (TCID₅₀) assay. B, Two groups of 3 guinea pigs were challenged with GA-SUDV and treated with 150 and 300 mg/kg/day of T-705, starting 2 days after infection and continuing every day up to day 8. An additional group of 3 animals were treated with meglumine instead of study drug. Blood samples were collected 5 and 9 days after infection. Animals were euthanized and tissue samples were harvested 9 days after infection. SUDV genome equivalent (GEq) RNA levels were detected by reverse transcription–quantitative polymerase chain reaction analysis in blood, liver, spleen, kidney, and lungs. Statistical comparisons between control-treated and T-705–treated guinea pigs were done by 1-way analysis of variance with the Bonferroni multiple comparison correction. *P < .05 and **P < .01.