Figure 10.

Effect of cdk4/6 inhibitors on VP40-producing cell viability and extracellular vesicle biogenesis. 293T and V2CH cells were treated with low, medium, and high concentrations of Fascaplysin (0.1, 0.5, 1.0 µM) or Ribociclib (0.1, 1.0, 10.0 µM) for 5 days. DMSO was also used at a final concentration of 1%. (A) Supernatants were harvested, filtered (0.22 µm), and analyzed by ZetaView for concentration of particles. (B) Cell viability was also measured by CellTiter-Glo. Statistical analysis by Student’s 2-tailed t test compares drug-treated groups to untreated controls of their own cell type (*, P < .05; **, P < .01; ***, P < .001).