Figure 4.

Blocking calcium (Ca²⁺)–dependent signaling reduces annexin V exposure during Ebola virus (EBOV) infection. The addition of calcium chelator (BAPTA) was used to block calcium-dependent translocation of PtdSer; findings shown represent 1 of 2 independent experiments. A, The extent of PtdSer translocation analyzed by confocal microscopy (scale bar represents 10 μm). B, Histograms depicting relative annexin V levels analyzed by flow cytometry. Gate shown was used to compare expression levels. C, Graphic representation of the percentage of annexin V⁺ cells from flow cytometry samples in panel B. Mean values are shown for triplicate samples, with standard deviations. ^*P < .01 (Student t test). Abbreviation: DAPI, 4′,6-diamidino-2-phenylindole.