Figure 5.

Blocking transmembrane protein 16F (TMEM16F) function with R5421 reduces viral infectivity. A, Huh7 cells were treated with an increasing concentration of R5421 to determine whether blocking TMEM16F reduces plasma membrane-associated phosphatidylserine after Ebola virus (EBOV) infection. Cells were incubated for 48 hours, stained with annexin V and analyzed by flow cytometry. B, Percentages of cells expressing green fluorescent protein (GFP) at 48 hours after EBOV infection of mock-infected or R5421-treated Huh7 cells. C, Viral production from R5421-treated Huh7 cells was determined by adding cell-free supernatants to Vero E6 cells. At 20 hours after infection, cells were fixed and analyzed for GFP expression by flow cytometry. Means shown in graphs are from triplicate samples, with standard deviations; results are representative of 3 independent experiments. ^*P < .01; †P < .001. Abbreviation: NS, not significant (Student t test).